13 puppies were seized at Dublin Port yesterday evening.
The 11 females and 2 males are a mixture of Rottweilers, terriers and other small breeds and were destined for the UK market.
Customs, Gardaí and the DSPCA were all involved in the operation.
The DSPCA says the puppies will not be rehomed before Christmas.
It says despite its messages and the previous seizures, those involved in the illegal puppy trade are ignoring the laws, attempting to smuggle dogs and puppies out of Ireland without the correct paper work or vaccination records.
Anyone wishing to adopt a puppy can visit www.dspca.ie/AdoptaPet or email foster@dspca.ie